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Additional resources for A textbook of inorganic chemistry vol.XI part II Organometalic compounds. Derivatives of Phosphorus, Antimony and Bismuth
It is uncommon that the cell biology department is dedicated solely to providing standard cell lines for consumption by screening groups. With increased focus on cell-based screening there is pressure to constantly develop and modify cell lines to address the current business strategy. With a manual or semi-manual approach it may take several months to develop a suitable cell line that is ready for standard production. More complex and ﬂexible research-scale automation is on the horizon, that may prove to be the key to optimizing cell culture conditions at small scales that are representative of large scale production .
First, the compounds must be compatible with compound handling and long-term storage conditions in solution and comply with the screening technology at hand. Second, the screening deck must contain compounds with biological relevance and that can modulate the target of interest. Third, the compounds must be amenable to lead optimization. There are considerable synergies between the requirements of technology compatibility and lead optimization. Water solubility is required not only for optimization but also to achieve the desired concentration in the assay system.
A central LIMS system keeps track of the compounds, layout of plates supplied from compound management and the assays requested for each sample. Scientists track the mapping of samples though the preparation of test plates, sample preparation and analysis with the help of macros (usually programmed in Excel). At the conclusion of the experiment, data is uploaded back into the LIMS system for review and delivery to the requesting scientist. Depending on the degree of automation, scientists may be preparing test plates and running the experiments manually, or operating preprogrammed liquid handler workstations.