By Xiaoshi Wang
In this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly stumbled on heme-thiolate peroxygenase, AaeAPO. This enzyme classification comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings makes a speciality of an extracellular P450 enzyme which isn't constrained in its balance and absence of solubility and accordingly is appropriate for common commercial use. the writer demonstrates that the peroxygenase catalyzes a variety of reactions. from time to time the writer even describes very tricky alterations in molecules which are hugely inert. Her exact investigations offer a mechanistic framework for the way the peroxygenase catalyzes this kind of huge variety of reactions. a tremendous spotlight of this thesis is the id of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing speedy kinetic and spectroscopic equipment, in addition to the elucidation of the thermodynamic homes of those high-energy intermediates. This paintings provides new perception into an immense classification of enzymes.
Read Online or Download A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry PDF
Similar chemistry books
Content material: Reassessing polymer lifetime prediction tools with more advantageous characterization and diagnostics / Robert Maxwell . .. [et al. ] -- getting older complexities brought about by means of the organotin catalyst in foamed polysiloxane elastomers / Mogon Patel, Steve Black, Julian Murphy -- Acceleration elements for the oxidative getting older of polymeric fabrics / Roger A.
The rivers run into the ocean, but the ocean isn't really complete Ecclesiastes what's quantum chemistry? the easy solution is that it's what quan tum chemists do. however it needs to be admitted, that during distinction to physicists and chemists, "quantum chemists" appear to be a slightly ill-defined class of scientists.
- Deoxy Sugars
- Medicinal Organometallic Chemistry
- Anorganische Strukturchemie, 6. Auflage
- Explosivstoffe. Zehnte, uberarbeitete und erweiterte Auflage
Extra resources for A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry
The crystal structure of chloroperoxidase: a heme peroxidase-cytochrome P450 functional hybrid. Structure 3, 1367–1377 (1995) 32. : Examining the role of glutamic acid 183 in chloroperoxidase catalysis. J. Biol. Chem. 278, 13855–13859 (2003) 33. : Crystal structure of H2O2-dependent cytochrome P450(SP alpha) with its bound fatty acid substrate insight into the regioselective hydroxylation of fatty acids at the alpha position. J. Biol. Chem. 286, 29941–29950 (2011) 34. : Hydrogen peroxide dependent monooxygenations by tricking the substrate recognition of cytochrome P450(BS beta).
Fundamentals and applications. Springer, Berlin (2011) 52. : Biological applications of raman spectroscopy, vol. 2. Wiley, Canada (1988) 53. : X-ray absorption spectroscopy. W. ) Nitrogen ﬁxation: methods and protocols, vol. 766, pp. 165–176 (2011) 54. : NMR spectroscopy in inorganic chemistry. Oxford University Press, Oxford (2000) 55. : Theory of magnetic circular dichroism. J. Chem. Phys. 52, 3489–3516 (1970) 56. : Principles of protein X-ray crystallography, 3rd edn. Springer, Berlin (2006) 57.
Our results suggest that AaeAPO could be a useful biocatalyst to prepare pharmacologically relevant drug metabolites and for the discovery of new commercial products including drugs. Clopidogrel (Plavix) is a prodrug metabolized by cytochrome P450 to an active form that inhibits ADP-induced platelet aggregation. Before clopidogrel, its analog ticlopidine was used in patients. Early studies have showed that among all of metabolites of ticlopidine, 2-oxo-ticlopidine was the only metabolite that had anti-platelet activity in vivo .