Download A Novel Heme-Thiolate Peroxygenase AaeAPO and Its by Xiaoshi Wang PDF

By Xiaoshi Wang

In this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly stumbled on heme-thiolate peroxygenase, AaeAPO. This enzyme classification comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings makes a speciality of an extracellular P450 enzyme which isn't constrained in its balance and absence of solubility and accordingly is appropriate for common commercial use. the writer demonstrates that the peroxygenase catalyzes a variety of reactions. from time to time the writer even describes very tricky alterations in molecules which are hugely inert. Her exact investigations offer a mechanistic framework for the way the peroxygenase catalyzes this kind of huge variety of reactions. a tremendous spotlight of this thesis is the id of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing speedy kinetic and spectroscopic equipment, in addition to the elucidation of the thermodynamic homes of those high-energy intermediates. This paintings provides new perception into an immense classification of enzymes.

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Extra resources for A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry

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Our results suggest that AaeAPO could be a useful biocatalyst to prepare pharmacologically relevant drug metabolites and for the discovery of new commercial products including drugs. Clopidogrel (Plavix) is a prodrug metabolized by cytochrome P450 to an active form that inhibits ADP-induced platelet aggregation. Before clopidogrel, its analog ticlopidine was used in patients. Early studies have showed that among all of metabolites of ticlopidine, 2-oxo-ticlopidine was the only metabolite that had anti-platelet activity in vivo [24].

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